Phytochemical and antioxidant screening of Gloriosa superb L. from different geographical positions of South India
Gloriosa superba Linn. is one of the important medicinal plant now in endangered list. This plant is widely used for several ethano-medicinal purposes by tribal peoples and traditional practitioners. Seeds and tubers contain valuable alkaloids viz., colchine and colchicoside as the major constituents, which are used an antidote for snake bites, gout and rheumatism. Present study was to evaluate the phytochemicals present in leaves, flowers, seeds and tuber samples were collected from four different places of South India Preliminary phytochemical and antioxidant Screening were carried out by standard procedures. Methanol extract of all samples were evaluated for protein, starch, total sugar and total phenol. The antioxidant potential of the extracts was assessed by employing different assays such as DPPH, ABTS, and Superoxide scavenging capacities. The screening tests also were performed for the presence of the following secondary metabolites such as alkaloid, flavonoids, phenols, steroids, tannin and terpenoids. The results were revealed the presence of various classes of compounds in different parts of the plant. Among the five different geographically collected plant samples (Six accessions), the Bhavani (BHA) sample showed the maximum amount of bioactive compounds and antioxidant activities than the other samples. The result of the extract from the tuber and seed samples were yields high amount of various biologically active compounds than the leaves and flowers. These compounds could serve as potential source for traditional medicines. Further research on this plant for the specific part could be used for isolation and characterization in large scale production.
Fig.: The Superoxide free radical scavenging activity of methanol extract of Gloriosa superba. Values are means of five replicate determinations (n =5) ± standard deviation.