International Journal of Botany Studies

ISSN: 2455-541X

Vol. 2, Issue 1 (2017)

In vivo and in vitro studies in Cymbopogon citratus (DC.) Stapf for sustainable development

Author(s): Vyshali P, Manjunath DR, Shiva Kameshwari MN, Thara Saraswathi KJ
Abstract: The Cymbopogon grasses belong to the family Poaceae yielding essential oil of commerce. The Cymbopogon citratus (DC.) Stapf presently investigated is commonly called as West Indian Lemongrass yielding Lemon grass oil. The essential oil is rich source of terpenoids having high Neral and Geranial content. During the study, the accessions of Cymbopogon citratus (DC). Stapf were collected from various geographical locations of south India and were maintained in the Departmental garden with an aim to restore the germplasm which existed as “cultigens”. The collected germplasm were predominantly found in south canara regions in Karnataka besides Kerala and Tamil Nadu. The collections were assigned with accession numbers and were subjected to cytogenetic and essential oil studies. The accession collected from Bangalore with robust habitat possessing high essential oil yield was used for further investigations. The karyological analysis carried out using root tips showed chromosome number of 2n=6x=60 from which the ideogram was constructed. The essential oil extracted from the plant was analyzed using Gas chromatography and 1% (v/w) yield was obtained showing Citral (72%) and Geraniol (17%) as predominant compounds. The in vitro culture studies performed using leaf explant revealed organogenesis and embryogenesis callus formation which were cultured further to obtain plantlets. Further, the rooted plantlets were acclimatized and transplanted to field conditions. The field established plants were maintained for further investigations.
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