International Journal of Botany Studies


ISSN: 2455-541X

Vol. 2, Issue 3 (2017)

Antibacterial and anatomical study of two Allium sativum L. Cultivars

Author(s): M Ishtiaq, Aneeqa Ishaq, Muhammad Siddique, Mehwish Maqbool, Shehzad Azam, Muhammad Shahzaman, Azhar Azam, Asif Nadeem
Abstract: Garlic (Allium sativum L.) plant is medicinally very important and is being used since time immemorial. In this research antibacterial activity and anatomy of two cultivars were tested by using dedicated methods. Diffusion method was used to test the antibacterial activity of the bulb of two different garlic species i.e. Desi garlic (DG) and white garlic (WG) against different bacteria such as (S. aureus, B. sibitils, E. coli, P. aeruginosa). The ethanol, methanol, chloroform, and distill water extracts of bulb of both species has tested which showed significant antibacterial activities on both gram positive and gram negative in contrast to white garlic. DG showed maximum antibacterial activity in chloroform and methanol extracts against E. coli and S. aureus. The chloroform bulb extract of DG showed maximum activity against E. coli that was 36.33±0.28. The methanolic bulb extract of DG showed maximum activity against S. aureus that was 32.33±0.571. The chloroform bulb extract of DG showed less inhibitory effect against P. aeruginosa that was 11.58±0.02. The chloroform bulb extract of WG showed maximum activity against E. coli that was 27.67±0.04. The methanolic bulb extract of WG showed less activity against P. aeruginosa that was 9.33±0.57. For anatomical study two samples of Allium sativum L. were collected from two different sites of District Bhimber. the DG (Allium seativum L.) stomatal size of Bandala showed maximum size as compared to DG (Allium sativum L.) stomatal size of Bhimber. The maximum stomatal size of DG of Bandala was 9.41±0.74um and the minimum size of DG of Bhimber was5.71±0. 74um.As it is WG (Allium sativum L.) stomatal size of Bandala showed maximum size as compared to WG (Allium sativum L.) stomatal size of Bhimber. The maximum stomatal size of WG of Bandala was 6.67±0.72um and the minimum stomatal size of WG (Allium sativum L.) was2.55±0.85um. The DG (Allium sativum L.) stomata index of Bandala showed maximum stomata index as compared to DG (Allium sativum L.) stomata index of Bhimber. The maximum stomata index of DG of Bandala was 97.74 and the minimum stomata index of DG of Bhimber was 92.89. As it is WG (Allium sativum L.) stomata index of Bandala showed maximum stomata index as compared to WG (Allium sativum L.) stomata index of Bhimber. The maximum stomata index of WG of Bandala was 85.63 and the minimum stomata index of WG (Allium sativum L.) was 47.33. Anatomical studies showed changes in stomatal size and index in collected species. It was concluded that DG extract was better than WG because former had stomatal index was higher than later and same depicted higher antibacterial activity than others, that is due to presence of more quantity of bio-constituents in taxa with numerous stomata.
Pages: 55-58  |  1457 Views  665 Downloads
download hardcopy binder
library subscription