Vol. 2, Issue 6 (2017)
Impact of organic farming on Spilanthes acmella Linn. an endangered important medicinal plant raised through tissue culture technique
Author(s): Nelofar Gulam Nabi, Mukta Shrivastava, Asrar Amin Khan, Abid Rashid
Abstract: Spilanthes acmella Linn belongs to family Asteracea is a multipurpose medicinal herb of tropical and subtropical regions. Due to its versatile properties the plant is in tremendous demand but due to its overexploitation and poor seed germination ability it has been depleted in its natural environment. An alternative method for its conservation and efficient rapid mass propagation is thus need of the hour. Therefore, the present work was aimed to develop an efficient in vitro propagation protocol for its rapid and large scale production. Different combinations of growth regulators in MS media were used to promote its shoot, and root formation. Maximum shoot proliferation was found in 0.5 mg/l of BAP in combination with 0.5 mg/l IBA. Regenerated shoots were rooted on MS medium supplemented with different concentration of auxins. The maximum rooting 90.5 % with 6.4 cm of root length was observed in MS media fortified with 0.5 mg/l IBA after 2 weeks of inoculation. Present study will help to propagate the medicinal plant for industrial herbal extraction of valuable pharmaceutical substances to be used in different diseases. This study will also help to execute further research on regeneration, transformation and hairy root induction of this important medicinal plant. The present research also focused on the methods and benefits of organic farming of the medicinal plant prevalent nowadays, although more better and profitable techniques can be introduced by carrying out further research in this regard. Hence it concludes that application of organic and biofertilzers acts as substitute of inorganic fertilizers in order to grow the medicinal and aromatic plants, should not be considered as a simple objective and short term benefits, but as a mean to improve the environmental conditions and human health. Therefore the present investigation was undertaken with the aim to prescribe the panacea by developing an effective method for rapid and large scale multiplication with protocol for effective organic farming to boost the vigour and other quantitative traits of the plant.