Vol. 3, Issue 1 (2018)
Phytochemical screening and antimicrobial efficacy of green synthesized silver nanoparticles from Neolitsea sericea (Blume) Koidz
Author(s): Srividhya Pattabiraman, Sinthia Ganesan, Thilagam Muthunathan, Palanichamy Mehalingam
Abstract: The present study we reported for the synthesis of AgNPs using Neolitsea sericea (Blume) Koidz. Leaf extracts were used as reducing and capping agent from silver nitrate solution. The green synthesis of silver nanoparticles was done by the bio reduction of silver nitrate using the plant extracts made from the leaves of Neolitsea sericea. Hence, the present study was focused to control the Gram positive strains such as Bacillus subtilis, Staphylococcus aureus, Streptococcus faecalis and Gram negative strains such as Klebsiella pneumoniae Escherichia coli, Pseudomonas aeruginosa and a fungus Candida albicans by screening their antimicrobial activity and synthesizing the silver nanoparticles. Subsequently this plant was screened to evaluate the phytochemical activities due to their high medicinal value. The dried leaves were extracted with ethanol. The extracts were subjected to various phytochemical tests. The test confirmed the presence of various phytochemicals in plant extracts. The synthesized leaf extract based silver nanoparticles were confirmed by the colour change from pale yellow to dark brown appearance. UV – visible spectroscopy studies were carried out to assess the formation of silver nanoparticles. Further, efficient antimicrobial activity of the synthesized silver nanoparticles proves the potential application of green synthesis in the area of nano-medicine.