Vol. 3, Issue 1 (2018)
Protein and isozyme analysis in different species, varieties and populations of Tabernaemontana
Author(s): Dipu Samanta, Sandip Mukhopadhyay
Abstract: Tabernaemontana is a member of Apocynaceae. Approximately 100 species of this genus are widely distributed in tropical parts of the world. Phytochemical studiesrevealed that in T. coronaria 66 different alkaloids have been identified from different parts. 22 types of alkaloids have been isolated from root and bark of T. dichotoma. The present investigation includes total protein analysis and study of isozyme activities showing distinct variations. Total protein analysis, both quatitative and qualitative, showed significant variations. Two isozymes indicated variations at species and variety levels. Proteins and isozymes are significant and widely used to study genetic diversity at interspecific and intraspecific levels. The number and position of isozyme bands differed in two species of Tabernaemontana and three varieties of T. coronaria. The differential distribution of both esterase and peroxidase activities has revealed genomic diversity among the species and varieties of Tabernaemontana. The differences among the populations are very low indicating their vegetative propagation.