Vol. 3, Issue 2 (2018)
High frequency plant regeneration through adventitious multiple shoot organogenesis in epicotyl explants of sensitive plant (Mimosa pudica Linn)
Author(s): Ramesh S, Chandran C, Karthikeyan K
Abstract: In vitro rapid propagation was deliberated from shoot tip, nodal and epicotyl explants of Mimosa pudica L. The explants were cultured on (MS medium supplemented with B5 vitamins (Murashige and Skoog 1962). In various concentrations of Cytokinins and Auxins ranging from 0.1mg to 3.0mg combinations of BAP, KIN, was good response from shoot tip, nodal, and epicotyl explant. Root induction of explant 2.0 mg IBA and IAA 2.5 mg. Highest number of callus induced in the concentrations of 2.5mg BAP 2mg NAA and 2-4D 0.5mg. Multiple shoot was noticed in 2.5mg BAP and KIN 1.5mg NAA 3.mg and 2mg IBA and shoot elongation in GA3 1.0 mg for good response after transplantation of gardening. The present study enables the large scale production of Mimosa pudica L. using in vitro conditions and disease free plants.