Vol. 3, Issue 2 (2018)
An investigation of enzyme inhibitory activity of different leaf extracts of Taxus baccata Linn.
Author(s): Ved Prakash, Shelly Rana, Anand Sagar
Abstract: Around 80% of the world’s population utilises traditional herbal medicines as stated by World Health Organization (WHO). Use of herbal remedies is on the rise in developed as well as developing countries. The current study was undertaken to investigate the leaf extracts of Taxus baccata Linn. For their enzyme inhibitory activity (α-amylase and urease) in three different solvents i.e. methanol, acetone and aqueous. The plant showed good α- amylase enzyme inhibition (57.40±2.12) at a concentration of 1 mg/mL in methanol extract whereas acetone and aqueous extracts showed moderate inhibition against α- amylase (46.30±0.66 and 37.90±0.80 respectively). In case of urease inhibition, methanol extract exhibited best inhibitory activity (52.40±2.10) followed by acetone and aqueous extracts at a concentration of 1 mg/mL. The plant extracts showed concentration dependent inhibition of α- amylase and urease enzymes. The inhibitory activity increased with increasing the concentration of each plant extract in the range of 0.2-1.0 mg/mL. The results further indicated that methanol leaf extracts exhibited maximum inhibitory effects than other solvent extracts. This tends to show that the active metabolites of the different plant parts are better extracted with methanol than other solvents. Therefore, the leaf extracts of T. baccata can be selected for further investigation to determine their ultimate therapeutic potential.