Vol. 4, Issue 1 (2019)
Computational Recognition and Characterization of EST-SSR in Vinca rosea
Author(s): Manisha Ray, Bhabani Shankar Sahoo, Souvagyalaxmi Sahoo
Abstract: Vinca rosea (Catharanthus roseus), normally known as Sadabahar; is a decorative plant in addition to having effective medicinal values, belongs to the family Apocynaceae. The expressed sequence tags (EST) are regarded as potential source for the development of genic microsatellite markers, gene discovery, comparative genomics and genomic studies. Thus this study conveyed to analyze the EST sequences from dbEST of NCBI for Vinca rosea, which had been assembled in CAP3 program after which analyzed in SSR detection tool GRAMENE and outcomes 20 numbers of SSR-EST sequences were subjected to functional annotation through InterProScan. The SSR-ESTs successfully designed 48 numbers of primer pairs by using Primer3. These findings probably beneficial to investigate molecular markers which have useful importance and have to additionally facilitate the evaluation of genetic variety in apocynaceae family especially in Vinca rosea species.