Vol. 4, Issue 6 (2019)
Screening of wheat (Triticum aestivum L.), genotypes under osmotic stress at germination and seedling stage.
Author(s): Hayat Ullah, Badshah Hussain, Noor Muhammad, Nisar Uddin, Niaz Ali
Abstract: Drought stress drastically decreases the yield of various crops including wheat. Germination stage is the most critical stage that is badly affected by drought stress. To screen out the wheat genotypes for osmotic stress, stress was induce by PEG-6000 to identify drought tolerant wheat genotypes after passing through screening criteria at seedling stage in wheat by using Petri dish. Twenty seven wheat genotypes were studied for seed germination percentage, germination rate index, root/shoot fresh and dry weight and root/shoot length. Relative net decrease was (64.9%). In germination rate index (GRI), (61.52%) germination, (91.64%), shoot length (SL), (91.13%) root length, (67.06%) shoot dry weight and (87.90%) in root dry weight was recorded under drought treatment after comparison all genotypes with control condition. In present in vitro experimental studies there was no germination in osmotic induce PEG (6000) treatment for genotypes (9409) PTSS08GHB00011S-0SH-099SHB-099SHB-52Y-0Y, (9414), PTSS08GHB00011S-0SH-099SHB-099SHB-89Y-0Y, (9418) SKOKOLL/WESTONIA and (9422) PUB94.15.12/WBLL1 respectively, therefore showed 100% decrease value in germination and badly affected by osmotic stress.