Evaluation of genotypic and hormone mediated callus induction and regeneration in sugarcane (Saccharum officinarum L)
Mudassar Fareed Awan, Muhammad Shahzad Iqbal, Muhammad Nauman Sharif, Bushra Tabassum, Muhammad Tariq, Shahid Murtaza, Sajed Ali, Amir Raza, Syed Ali Raza Bukhari, Idrees Ahamd Nasir
Eight elite sugarcane cultivars i.e., HSF-242, HSF-240, CP-43, CPF-237, CPF-238, SPF-245, CPF-213 and SPF-234 were evaluated in response to different hormones for callus induction and regeneration. Leaf sheaths used as explant. Various levels of 2,4 D, NAA and IBA were exploited to induce callus and regenerate sugarcane callus of selected genotypes. Likewise, Cytokinin and Auxin were provided in disparate quantities to initiate shoot and root development from regenerated callus. The optimum level of callus formation was induced in variety HSF-242 was 96% and 91%. It was observed that 2,4 D and IBA should be maximum at 3.0mg/l and 2.5mg/l respectively. The hormone NAA has shown zero effect on callus formation. IBA was not largely effective for callus induction. Embryogenic callus percentage was seen to be maximum in sugarcane genotype HSF-240. Similarly, maximum level of regeneration was also observed in HSF-242 stands at 91%. Shoot regeneration in all selected sugarcane varieties proved to be maximum at 1.0mg/l for both BAP and kinetin hormones. Rooting of the sugarcane plantlets was also established on half strength MS medium. Percentage of rooted shoots was optimal at 3.0mg/l for all auxin hormones.