Vol. 5, Issue 1 (2020)
Endophytic fungal associations and VAM infection in the roots of Murraya koenigii (L.) Spreng. from the Hamirpur Distt of Himachal Pradesh
Author(s): Dr. Jaya Thakur, Anand Sagar
Abstract: The impact of fungal endophytes on the leaves, bark, stem and root and vesicular arbuscular mycorrhizal fungal infection in the roots of Murraya koenigii was investigated. It has been found that both endophytic fungi and VAM fungi played an important role in the growth and development of the plant. Endophytic fungal species were isolated through three step method. Five species of endophytic fungi belonging to 4 genera (Aspergillus, Alternaria, Fusarium and Trichoderma) were isolated. The main species were Alternaria alternata, Fusarium oxysporum, Fusarium moniliforme, Aspergillus niger, Trichoderma viride isolated from the leaves, bark, stem and roots of Murraya koenigii. Aspergillus and Fusarium belong to Ascomycotina and Trichoderma and Alternaria alternata belong to the Deuteromycotina. The VAM fungal infection in roots was also assessed by using Phillips and Hayman (1970) technique. Vesicles and fungal hyphae (mycelium) were observed in the roots of Murraya koenigii.