Vol. 5, Issue 2 (2020)
Anti: Proliferative efficacy of the leaf extract of Aristolochia tagala cham, on hep G2 cell line
Author(s): GS Shailaja Sharma, L Rajanna
Abstract: Aristolochia tagala Cham. is an important medicinal plant used to treat tumors in ethno botanical Practices. During the present investigation, dried leaf powder of A. tagala was extracted with three different solvents by decoction method and were subjected to MTT cytotoxicity assay against HepG2 – human liver carcinoma cell line. Methanolic leaf extract showed best anti-proliferative activity against HWepG2 cell line with an IC50 value of 80 μg/ml than other two extracts. Cell cycle arrest was studied by PI staining and the treated cells were arrested at G2/M phase. Apoptosis was assessed by annexin-V and PI staining, the results revealed high annexin-V and low PI staining representing early apoptotic stages and finally Caspase-3 expression in the treated cells were analyzed by using flow cytometry having negligible caspase-3 activity hinting for a caspase-3 independent pathway leading to cell death. In the present study methanolic leaf extract of A. tagala showed a competitive anti-proliferative and apoptotic activity against HepG2 cell line giving a partial scientific validation for its usage in traditional system of medicine. Further the affirmative results call for advanced studies on this medicinally important plant is needed.