Vol. 5, Issue 2 (2020)
Detailed micrometrical, pharmacognostical and analytical evaluation of Rhododendron arboreum Sm. leaves
Author(s): Akanksha Thakur, Harisha CR, VJ Shukla, BR Patel
Abstract: Rhododendron arboreum Sm. is an ethnomedicinal plant of Himachal Pradesh belonging to family Ericaceae. It is locally known as Barah, Brass and its leaves are used in the treatment of fever, headache, diarrhoea, gout and rheumatism. The present study is designed to investigate the pharmacognostical profile of leaves including powder microscopy, detailed micrometric evaluation and analytical study. Transverse section of petiole is circular to heart shape in outline showing central arc shaped vascular bundle and one - two meristeles on its either side. Transverse section of leaf shows upper epidermis and lower epidermis followed by collenchyma, ground tissue, central vascular bundle. Covering trichomes and sessile glandular trichomes are present on lower surface. Leaf powder shows the presence of trichomes, epidermal cells, stomata and rosette crystals. Loss on drying of leaves found to be 4.29 ± 0.16 % w/w. Qualitative tests showed the presence of tannin, alkaloids and flavonoids. HPTLC study reveals presence of 9 and 8 peaks at 254 nm and 366 nm respectively. This study would be useful in the identification and authentication of the plant material.