Illustration the genetic diversity among ten accessions of Tephrosia collected from KSA using three molecular markers (RAPD, ISSR & SCoT). Molecular markers produced 99 total bands with 20 monomorphic bands and 79 polymorphic bands. Molecular data generated from RAPD –PCR showed highest polymorphism (92.31%) using ten primers with 10 unique bands. SCoT-PCR marker showed the lowest polymorphism (56.52%) with one unique band. RAPD markers may be more efficient in studying genetic diversity and relationship of Tephrosia than ISSR and SCoT markers with regards to polymorphism detection. This result could be explained as RAPD marker covers the whole genome for amplification. Cluster dendrogram and Biplot give the possibility to separate the studied taxa into two groups; first group include only of Tephrosia purpurea ssp. leptostachya and the second group contain other nine accessions. These results are important to improve the accuracy of resolution of genetic relationships for sustainable conservation as medicinal plant by such as the construction of gene banks of the genus Tephrosia.