Vol. 5, Issue 2 (2020)
Action of Berberis aristata on various aspects of bone remodeling
Author(s): Kunjal L Vegad, Nimisha P Kakadia, Niranjan S Kanaki
Abstract: Context: Osteoporosis is an age-related bone disease in which bone mineral density is decreased which may lead to fracture with minimal trauma or without severe stress to bone. Several herbs have been used in Ayurveda for treatment of bone fracture. Berberis aristata (BA) stem bark, traditionally known as Daruharidra, is one such drug used in Ayurveda for bone-related disorders. Aim: The aim of the study was to examine the effects of B. aristata stem bark on various aspects of bone-remolding using in-vitro cell-based assays. Materials and Methods: The effect of alcoholic and aqueous extracts of Berberis aristata on proliferation of primary bone marrow cells (mesenchymal stem cells) was studied using MTT assay. The stimulation of differentiation of primary bone marrow cells into osteoblasts was assessed by measuring ALP activity, which is marker of osteoblast activity. The inhibition of osteoclast formation by the extracts was assessed by quantifying the TRAP activity. The deposition of calcium in extracellular matrix by osteoblasts was observed under microscope after formation of an orange-red complex of calcium with alizarin red S. Statistical analysis used: Statistical analysis of the data was performed by one- way ANOVA followed by Tukey’s multiple comparison test Results: The aqueous extract of stem bark of Berberis aristata was found to stimulate the differentiation of primary bone marrow cells to osteoblasts, to promote the deposition of calcium in extracellular bone matrix and to inhibit the formation of osteoclasts. Conclusions: Berberis aristata stem bark acts on various aspects of bone remodeling, justifying its ability to restore equilibrium between bone formation and resorption in osteoporosis.