Vol. 5, Issue 3 (2020)
Isolation and molecular characterization of plant growth promoting fungi from rhizosphere soil
Author(s): Jyothimol Joy, Haritha M, Meenu Devassy, Nivya Mariam Paul
Abstract: Plant growth-promoting fungi (PGPF) have attracted ample interest as bio-fertilisers due to their multiple advantageous outcomes on plant quantity and quality. In this study, the potential of rhizosphere fungi is revealed. Samples were collected from the rhizosphere of rubber plant at Kothamangalam. Microorganisms were isolated by serial dilution method and further inoculating in SDA media for fungal growth. Pure colonies were isolated by repeated subcultures. Enzyme production assay was checked with the isolated fungi and it showed the production of amylase, cellulase, protease and lipase enzyme. Molecular identification was performed by analysing sequences of the nuclear ribosomal RNA genes from internal transcribed spacer (ITS) region. This studied the phylogenetic relationship of the isolated samples by comparing the ITS sequences with those stored in the GenBank database. Among the isolated fungi, Penicillium species showed potential enzyme production and lipase enzyme production as greater for this fungus. So this Penicillium species can be used for the large scale production of lipase enzyme and also it can be utilized as a biofertilizer for agricultural uses.