Vol. 5, Issue 6 (2020)
Comparative study of Ashodhita and Shodhita seeds of Ipomoea carnea Jacq with respect to level of toxic compound (Swainsonine)
Author(s): Nilima Narayanrao Wadnerwar, Meena Shyamrao Deogade
Abstract: Background: Ipomoea carnea Jacq is an extra pharmacopoeal drug used as folk medicine. Swainsonine is the main toxic principle in it. Leaves and flowers of Ipomoea carnea contain Swainsonine, Calystegines B1, B2, B3 and C1 along with other phyto constituents. But seeds contain only Swainsonine, Calystegines B1, B2, B3 and C1 and are not used as medicines because of its highly toxic nature. It can be used after shodhana methods of purification mentioned in Ayurveda, if shodhana could reduce the percentage of Swainsonine present in seeds. Objectives: To study the effect of Shodhana of Ipomoea carnea seeds by using common shodhana media and to study the physicochemical and phytochemical changes and level of Swainsonine (the toxic principle) present in seeds before and after shodhana by HPLC. Methods: After identification and authentification, seeds of Ipomoea carnea will be divided in six groups each containing 10 gms and shodhana of seeds will be performed by following method of swedana (boiling) in dolayantra for three hours using four different shodhana media media viz. Godugdhha (Cow milk), Gomutra (Cow urine), Kanji (sour gruel), Triphala kwatha and Distilled water for each sample. The purified samples will be compared for the level of Swainsonine estimated with the help of HPLC with unpurified sample of Ipomoea carnea. Result: Physicochemical, pharmacognostic and phytochemical changes and effect on level of toxic principle (Swainsonine) of Ipomoea carnea seeds will be interpretated. Conclusion: Effect of shodhana on toxic compound (Swainsonine) level present in Ipomoea carnea seeds will be assessed on the basis of result.