Vol. 5, Issue 6 (2020)
Physio-morphological Variations induced by methyl methane Sulphonate in Trigonella foenum-graecum L
Author(s): Neha Naaz, Shazia Bi Ansari, Samiullah Khan, Sana Choudhary, Roshan Jahan
Abstract: Since age-old times, herbal therapeutics has been involved in conventional systems of medicine such as Unani and Ayurveda. Mutation breeding has involved advance genetic approach and recognition of ameliorating or novel phenotypes and their incorporation into breeding programmes. Fenugreek (Trigonella foenum-graecum L.), an important medicinal herb of the family Fabaceae with therapeutic importance and was preferred in the present work to examine the response of potent chemical mutagen, i.e., Methyl Methane Sulphonate (MMS) on physio-morphological traits in M1 generation. Germplasm (seeds) of two varieties of fenugreek was exposed to four mutagenic concentrations of MMS, i.e., 0.01%, 0.02%, 0.03%, and 0.04% contributing to the genetic dissection of the wild type genes. As the concentration of mutagen increased, it showed inhibitory effects on germination percentage, seedling height, plant survival, and pollen fertility. Total chlorophyll content exhibits both negative and positive shifts while negative correlation observed in carotenoid content from their control in both varieties. Scrutiny on quantitative attributes involving plant height, fertile branches/plant, number of pods/plant, number of seeds/pod and 1000 seed weight exhibit remarkable variations at different mutagenic treatments. This analysis revealed that lower and intermediate doses of mutagen exerted less biological damage and ameliorated the genotype of Trigonella foenum-graecum. Indeed, mutation facilitated crop breeding will play a pivotal role in the procreation of smart crop varieties and meet the challenges of the present and future exigency in medicinal crops.