Vol. 5, Issue 6 (2020)
Screening some medicinal plants for endophytic fungi and their antibacterial activity against some human pathogens
Author(s): R Anitha, M Sathiyabama
Abstract: The aim of the present study was to screen for antimicrobial activity in endophytic fungi isolated from surface sterilized leaves of some medicinal plants viz., Anisomeles malabarica, Cardiospermum halicacabum, Aristolochia indica, Acacia mangium. Totally 14 isolates were isolated from these plants. Preliminary analysis of fermentation broth of these isolates was tested for antimicrobial activity by agar diffusion method. Seven isolates displayed antimicrobial activity against at least one pathogenic bacterium. From these, one isolate from each plant was selected based on their morphotypes for further analysis. The isolates were identified using morphological/moleulcar methods (ITS) as Alternaria alternata, Alternaria sp., Rhodotorula mucilaginosa and Phyllosticta citricarpa. The crude ethyl acetate extract of A. alternata showed strongest antibacterial activity against the tested bacterial pathogens. These results indicate that A. alternata as a potential source of antimicrobial agent.