Microrhizome induction in Kaempferia galanga L. using silver nanoparticles

Vidya VR; Gopika K; Hemanthakumar AS; Padmesh P; Preetha TS

Microrhizome induction in Kaempferia galanga L. using silver nanoparticles

Authors
Vidya VR, Gopika K, Hemanthakumar AS, Padmesh P, Preetha TS

Abstract
Kaempferia galanga L. is an economically important medicinal plant used in several ayurvedic formulations and over-exploited to the extent that there is always scarcity of rhizomes that are the propagating material. The present study takes into account of the beneficial role of silver nanoparticles (AgNPs) on microrhizome production in this taxa. The concentration of AgNPs were varied (such as 12.5, 25, 50, 100 and 200 mgl^-1)in the Murashige and Skoog (MS) nutrient medium supplemented with 0.1 mgl^-1 TDZ and 2.0 mgl^-1 NAA and the different treatments were designated as T1, T2, T3, T4 and T5. Variable morphological features with respect to shoots and roots were noticed in the treatments compared to control. Comparatively short shoots were noticed in AgNP-treated plants. Roots were tuberous that intrinsically varied in their morphology in different concentrations of AgNPs. Induction of microrhizome was seen in all AgNP treatments irrespective of its concentration and it was at maximum intensity in T2 (25 mgl^-1 AgNPs). The synergistic effect of AgNP-treatment along with 6% (w/v) sucrose supplementation and dark period incubation hastened the partitioning of assimilates to the storage parts thereby causing the development of microrhizomes in K. galanga. The induction of microrhizoes was confirmed by the anatomical studies and it was compared with the rhizomes formed in the field (mother rhizomes). The outcome of this study can be further used for mass production of pathogen-free microrhizomes and conservation of K. galanga for its sustainable utilization.

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