Identification, isolation and characterization of Aphanamixis polystachya flavonoids by UV, IR and RP-HPLC

Neelam Dashputre; Shubhangi Sukenkar; Pavan B Udavant

Identification, isolation and characterization of Aphanamixis polystachya flavonoids by UV, IR and RP-HPLC

Authors
Neelam Dashputre, Shubhangi Sukenkar, Pavan B Udavant

Abstract
Objectives: The present study aims the phytochemical screening, isolation and characterization of isolated flavonoid fractions of the Aphanamixis polystachya (wall.) Parker stem bark extract, which is an important medicinal plant in all regions which could be further used as tool for standardization. Methods: Successive extraction was carried out by Soxhlet extraction and maceration method. Further all extracts were studied for phytochemical screening for presence of various phytoconstituents. Total flavonoid content and phenolic content determination with standard AlCl₃ colorimetric method and Folin-Cioacalteu assay performed for all extracts. Ethanolic extract subjected for the column chromatography and fractions were collected further subjected for qualitative analysis by thin layer chromatography (TLC), UV-Vis Spectrophotometry, RP-High performance liquid chromatography (HPLC) and IR interpretation performed for structural elucidation. Results: Phytochemical screening shown the presence of alkaloids, phenols, flavonoids, cardiac glycosides, saponin glycosides, anthraquinones, terpenoids and steroids. Total flavonoid and phenolic content were found highest in ethanolic extract. TLC, UV, HPLC and IR interpretation of isolated fraction confirmed the presence flavonoids when compared with of standard markers. Conclusion: Study findings confirmed that A. polystachya stem bark extracts have the presence of flavonoids which has wide range of human health benefits. This study opens up the new ways to exploit a particular plant for various therapeutic and restorative purposes and the study will help in future for identifying this plant for further research.

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