in vitro seed germination and histology of protocorm development in Spathoglottis plicata blume
Smitha P D, Geethu M S
Most terrestrial orchid seeds are difficult to germinate in vitro and exvitro condition due to its specific nutrient and environmental requirements. Present study aimed to investigate the germination of seeds, development and histology of protocorm. Tissue culture studies were carried out in MS medium with BA (0.1-2.00 mg/l), Kin (0.1-2.00 mg/l), TDZ (0.1-2.00 mg/l) or in basal MS medium. During sowing seeds in a culture medium, seed germination occurred after the imbibition, which resulted in swelling of the embryos, and then the seed coats were ruptured by the emerging of germinated embryos, so the embryo enlargement and the papillae appeared on the one end of protocorms after the second subculture. MS medium supplemented with BA (0.5 mg/l) resulted in 98% of seed germination within the third week of inoculation. Histological study revealed the presence of vascular tissue in the central region at early development and later developmental stages showed well-developed shoot and root pole. In the present study, we reported a simple, quick, structured, and dependable in vitro revival system for mass propagation of S. plicata through the culture of seeds. The protocol established in this species will facilitate in vitro propagation for commercial purposes, sustentation, and metamorphic studies.