Taxonomy and traditional medicinal uses on the family Apocynaceae growing throughout the Rajshahi district has been made. A total of 14 species under 12 genera belonging to the family Apocynaceae were collected and identified. Out of the total number of species Allamanda cathartica Linn, Alstonia scholaris (L.) R. Br., Carissa carandas Linn, Catharanthus roseus (L.) G. Don., Ichnocarpus frutescens (L.) R. Br., Nerium oleander Linn, Plumeria alba Linn, Plumeria rubra Linn, Rauvolfia serpentina Linn, Tabernaemontana divaricata Linn, Thevetia peruviana (Pers) K. Schum. were common and Cerbera odollam Gaertn, Holarrhena antidysenterica Linn, Rauvolfia tetraphylla Linn were rare species in the study area. For each species English name, botanical name, local name, status of occurrence, flowering season, distribution, voucher number and traditional medicinal uses have been mentioned. This information will be beneficial in public health, research and providing lead to plants that can be useful in drug discovery.