Trillium govanianum commonly called as “Nag chhatri” is an endangered plant species being traditionally over exploited by the tribal people to curve diseases like diarrhoea, dysentery, ulcerous wounds and menustrual and sexual disorders. Studies conducted on endophytes and antibacterial activity of this plant revealed that its leaves, stem and rhizome harbour nine genera of fungi (Alternaria, Aspergillus, Fusarium, Mucor, Phoma, Pythium, Rhizopus, Stachybotrys and Trichoderma as endophytes. The antibacterial activity of methanol, ethanol, acetone and distilled water rhizome extracts of T. govanianum was determined in vitro against three human pathogenic bacteria (i.e. Escherichia coli, Yersinia pestis and Staphylococcus aureus) following agar well diffusion method using different concentrations of plant parts extracts (25%, 50%, 75% and 100%). The screening revealed that methanol rhizome extract of T. govanianum was most effective in inhibiting the growth of S. aureus. There is a need to analyze the bio active molecules and secretions of endophytes present in this medicinal plant.