Seed shape, dimensions, surface texture and sculpture, Hilum shape and position were recorded for eleven species of Acanthaceae by using light microscope (LM), and scanning electron microscope (SEM). Seven patterns were recognized based on surface sculpturing pattern: reticulate undulate, papillate, rugose, heteromorphic tubercles, ruminate, moniliform cell, polygonal. Anatomical investigation using light microscope showed that the hypodermis is present in the outer integument of four species and absent in the rest. The inner integument is recorded two types. The data proved useful in the construction of a bracketed key to the species. The potential taxonomic value of the recorded characters is indicating by the richness of variation recorded in the limited sample of genera and species.