Callus induction was performed using young leaf base of Cymbopogon citratus (DC.) Stapf and was grown on MS media. Shoot and root regeneration with different hormones were tried like 2, 4-D (0.5-2 mg/L), BAP (0.5-1.0 mg/L), NAA (0.5-1.0 mg/L) and Kn (0.5-1.5 mg/L) in MS media. Methyl Jasmonate (MeJA) is used as a major elicitor for manipulating defense pathways which modifies the production of terpenoids by activating genes controlling the secondary metabolic pathways was given in varied concentrations (25, 50 and 100 µM/L). Later the analysis of secondary compound production was done using GC-MS which showed Citral as the main active component elicited in the cell culture. The percentage of Citral (50%) produced was higher in cell culture treated with 50 µM/L of MeJA.