The antimicrobial properties of acetone extract of Tectaria gemmifera (Fee) Alston rhizomes and leaves were assayed in vitro against human pathogenic bacteria represented by Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, Escherichia coli and Shigella flexneri by 96 well-plate method. Extracts exhibited varying degree of inhibitory effect against all tested pathogenic bacteria. Leaf extract shown maximum inhibition and minimum inhibitory concentration (MIC) for S. flexneri and P. aeruginosa (2 µl), S. typhi and E. coli (2, 6 and 8 µl) and S. arueus (10 µl). Whereas rhizome extract shown (MIC) of S. flexneri and P. aeruginosa (2 µl); S. typhi (6 µl); E. coli (8 µl) and S. arueus (10 µl). The antibacterial activity may due to presence of secondary metabolites in the plant. Phytochemical tests showed the presence of steroid, glycoside, flavonoid, tannin, phenol, terpenoids, phytosterol and saponin in rhizome while absent in leaf extract. The results of pharmacognostic analysis will be helpful in developing standards for purity, quality and identification of the crude drugs.