This paper presents the diversity based study of Closterium in Tapti pond from Multai, district Betul (M.P.). Closterium is a crescent – shaped or elongate desmid. Closterium is a genus of unicellular Charophyte green algae in the family Closteriaceae. Some species of Closterium are straight and needle like, but most are broader with curved ends like smile. The ends of the cell are usually tapered and may be pointed or rounded. This placoderm, unconstricted desmid is found in freshwater reservoirs like ponds, ditches, pools, streams etc, often mixed with some other free-floating algal members.