Jatropha curcas plant has tremendous scope in contributing growing demand of energy resources. It can grow in any kind of soil; therefore, will not compete with conventional crop for cultivation. For the large scale propagation, several regeneration systems have been published using different explants such as cotyledons, hypocotyl, epicotyls, stem, leaf, shoot apex, petiole, node, axillary buds etc. Using these regeneration systems, a novel gene can be introduced in Jatropha plant for improving biodiesel quality.