Holostemma ada-kodien is well known for its medicinal and rejuvenative potential. A preliminary phytochemical screening and estimation of total phenols in the leaf tissue was also carried out in the present study. The present finding of this study support the view that H. ada-kodien is a promising source of potential antioxidants which can be used in treatment of various diseases. The qualitative analysis of alkaloid, tannin, saponin, phlobatannin, flavonoid, terpanoid, glycoside, steroid, quinone, coumarin and phenol are done. And also the quantitative estimation of total amount phenols was done by using catechol as standard.