The present communication relates to the status and ethno-medicinal knowledge of the flora of world famous Rajaji National Park in the state of Uttarakhand (India). The paper describes local/traditional uses of the 135 medicinal flora representing 63 families, in which 45 species were trees, 58 were herbs, 24 were shrubs and 08 were climbers. Amongst these families, Poaceae was dominant (11 plant species) in having medicinal importance, followed by Caesalpiniaceae, Apocynaceae and Moraceae with 6 species each. Various parts, such as: whole plant (35), roots (24), fruits (23), stem (4), leaves (41), barks (23), rhizome (4), bulbs (2), buds (3), seeds (12), flowers (7), tuber (1) and bulbils (1) were used in different ailments. Of the 135 medicinal plants, 18 species were reported to be threatened, endangered or rare. These identified plants need conservation and protection.