Present study examined the general biosynthetic route leading to the formation of glycine betaine probably involves the sequential methylations of ethanolamine to choline followed by a two steps oxidation of choline to glycine betaine. The role of phosphotidyl choline and phosphotidyl ethanolamine in the biosynthesis of betaine raise the significance of betaine accumulation during salt stress in lentil whether the accumulated turn over of methylations phospholipids be an a adaptive response to salinity or betaine accumulation turn over of phospholipid head groups during stress.