Staurogyne spatulata (Blume) koord, belongs to the family Acanthaceae is a small pubescent herb. Scientific information on their pharmacognosy and phytochemistry is very scant. Hence the current study describes some pharmacognostical, physiochemical and phytochemical investigations undertaken on the stem, root and leaves of species. The herbs were subjected for morphological, microscopical, physiochemical, phytochemical and fluorescence analysis methods for standardization. The parameters from the above were recorded with an objective of drawing attention on the species as well as a reference for further scientific investigations.