The present study deals with in vitro antifungal activity of bioactive compounds present in leaves of Centella asiatica. Extracted with hexane, chloroform, ethyl acetate, and ethanol were tested against human pathogens, such as Apergillus flavus, Apergillus fumigates, Aspergillus niger, Candida albicans Candida glabrata, Candida tropicalis, Microsporium canis, Microsporium gypseum and Trichophyton rubrum by disc diffusion method. The phytochemical tests were revealed the presence of alkaloids, total phenols, glycosides, terpenoids, steroids, flavonoids, tannins, saponins and reducing sugars. The ethanolic extract showed maximum yield of bioactive compounds than the other solvent extracts. In the present investigation all the extracts were found to be effective against nine human fungal pathogenic species. The maximum inhibition was observed on Candida trophicalis (25.3 mm) with ethanol extract of leaves followed by Aspergillus flavus (24.4 mm), Candida glabrata (24.0 mm), Apergillus fumigates (23.5 mm), Aspergillus niger (22.2 mm), Candida albicans (21.5 mm), Microsprium canis (20.7 mm) and Microsporium gypseum (19.8 mm). The minimum inhibition was observed on Trichophyton rubrum (12.6 mm). The hexane extract of leaves also showed the promising antifungal activity against to all tested fungal cultures. The results were validating the traditional uses of this plant in treatment of various diseases. This kind of study could generate more such ideas for re-inventing and using herbs in combination to treat many more diseases.