The sunflower oil is deteriorate due to seed borne fungi. In the present study some seed – borne fungi were isolated from seeds of sunflower which were collected from different location which include Aspergillus flavus, A. niger, alternaria alternate, curvularia lunata, fusarium oxysporum, penicillum digitatum, f. semitectum for this agar plate method and standard blotter method are used several pathogenic fungi were also observed among all of observed fungi Alternaria speciaes were found in more amount in both the method among both method standard blotter method was found to be more better as compared to agar plate method.