Pogonatum microstomum belongs to family Polytrichaceae (Bryophyta; Musci). The phytochemical group test of three solvent extracts of this plant materials for the detection of secondary metabolites were performed by the standard methods. Flavonoids, glycosides, triterpenoids, phenols and sterols are present in all three extracts. Saponins present only in chloroform extract. Antimicrobial properties of various extracts was detected against two Gram – positive and seven Gram – negative human pathogenic bacteria and four dermatophytic fungi and it was determined by standard agar well diffusion method. The chloroform extract showed the higher degree of inhibition zone against, Streptomyces pneumonia, Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Agrobacteirum tumefaciens. Pet ether and chloroform extracts showed the higher degree of inhibition zone against Candida albicans and Trichophyton rubrum.