Poisonous plants comprise the third largest category of poisons known around world. An account of 41 poisonous angiosperms under 22 families occurring in Rohilkhand region of Uttar Pradesh (India) has been documented. In this survey, total nine districts were surveyed and collected the information of the poisonous plants that are grown, wild, planted, cultivated & naturalized of road sides, park avenues and gardens. The plants were identified with the help of different published floras and herbarium lodged in FRI & BSI, Dehradun and BSI Allahabad. The information on the poisonous plant’s species has been gathered from the local people during ethno-botanical field survey. The study suggests that the local people are not only aware of such poisonous plants and their harmful effects, but also use them to treat various ailments.