Micropropagational studies in Enicostema littorale
Present investigation aim to develop standard protocol for micropropagation of herbal drudge Enicostema littiralae. This perennial herb distributed in West Indies, tropical Africa, India and Sri Lanka. It is found almost throughout India up to an altitude of about 450 meter. The whole plant is used in curing from various diseases including endemic disease diabetes mellitus, very frequently throughout the India. It is also utilised in rheumatism, abdominal ulcers, hernia, swelling, itching, malaria and insect poisoning. Present investigation revealed that induction of callus and multiple shoots on MS medium supplemented with various concentration of growth regulator alone or in combination. Maximum callus induction frequency was achieved on MS media along with 2.0 mg/L of 2, 4 D alone with Somatic embryo and 2.0 mg/L of BAP in combination of 0.5 mg/L of IAA. Induction of multiple shoots was achieved on IAA in combination of BAP 0.5 mg/L and 1.5 mg/L. Standard protocol was established for micro propagation of herbal drugs using IAA, 2, 4 D, BAP and KIN.