In-vivo pharmacological investigation of root extract of Myrtus communis L. by using Animal model (Mus musculus)
Rahim Gul, Hikmat Ullah Jan, Muhammad Tayyab Gul, Muhammad Ali Khan, Ayesha Malik, Samin Jan, Rafiullah, Ali Sami Dheyab, Norhayati Muhammad
The Myrtus communis is traditionally used for curing skin irritation and intestinal diseases. Therefore the present study was aimed to investigate the effect of Myrtus communis root extract (MCRE) for analgesic activity by acetic acid-induced writhing test, and tail immersion test, carrageenan induced model was used for evaluation of anti-inflammatory activity while the antispasmodic activity by intestinal transit of charcoal meal. Analgesic activity of (10 and 15mg/kg) showed normal results (51.5%, 59.3% respectively) but higher dose (20 mg/kg) showed good result (70.3%) compared to diclofenac sodium. Antispasmodic activity showed that the extract was dose-dependent higher dose showed longer distance covered by charcoal in the intestine. The results of anti-inflammatory activity showed that only MCRE at 20mg/kg revealed the effect to lessen the mice paw edema at one hour of administration compared to aspirin. Hence it is proved that MRCE contains analgesic, anti-inflammatory and antispasmodic agents which support its uses in traditional medicine. However; further studies are required for identification and isolation of bioactive constituents of Myrtus communis.