Effect of growth regulators on In vitro organogenesis in cauliflower (Brassica oleracea L. var. botrytis)
Nasmin Banu CV, P Indulekha
Cauliflower (Brassica oleracea l. var. botrytis) is a member of family Cruciferae (Apiaceae), and is cultivated all over North and South India. The whole inflorescence forms a large head of flowers on thick hypertrophied branches which are eaten as vegetable. Cauliflower contains Sulforaphane, which was shown to kill cancer stem cells. It also relieves high blood pressure. It contains antioxidants like vitamin C, and beta carotene. Conventional propagation takes a long period for multiplication due to poor rate of fruit set; poor germination and heterozygosis through seeds. In vitro culture includes culturing of cells, tissues, organs under aseptic laboratory conditions in culture media. Plant parts known as explants are cultured in nutrient medium. Plant tissue culture can overcome these problems and establish plants successfully through a standardized protocol. MS (Murashige and Skoog, 1962) basal medium was used for callus culture and organogenesis. The Auxin and Cytokinin used for leaf explants were NAA (Naphthalene Acetic Acid) 3 mg/L and BAP (Benzyl Amino Purine) 0.5 mg/L. MS medium +NAA 3 mg/L and BAP 0.5 mg/L was proved to be a favorable medium for callus induction, proliferation and shoot organogenesis.