GC-MS screening of bioactive compounds from extracts of phylloplane fungi of Markhamia lutea (Benth.) K. schum
Rahat Ubaid Ansari
Phylloplane fungi are fungi growing on the surface of the leaf. Leaves forms a nutritive substrate for growth of fungi. Leaf and fungi grow in association with each other either symbiotically or symbiotically. Bioactive compounds are the frontline potent agents in both nutraceutical and pharmaceutical industries. Bioactive compounds gain higher importance in enhancing resistance to various diseases in plants as well as humans. (Dinesh Kumar et al., 2018). The present investigation was undertaken to explore the potential bioactive compounds present in the extracts of leaf of Markhamia lutea and their phylloplane fungi. Thus, the present study was focused mainly on the screening, analysis and comparison of these phytochemical and biochemical compounds using Gas chromatography higher resolution mass spectrometry after the isolation and identification of phylloplane fungi from leaves of Markhamia lutea. Aspergillus flavus, Aspergillus niger, Alternaria alternata, Colletotrichum gloeosporioides, Curvularia lunata, Fusarium oxysporum, Paecilomyces lilacinus, Penicillium sp., Phoma chrysanthemicola, Phomopsis sp., Rhizopus stolonifer, Trichoderma sp., Stachybotrys sansevieriae were the phylloplane fungi isolated from the leaf of Markhamia lutea. The GC-MS analysis of DCM, Ethyl acetate and Methanol extracts of Markhamia leaf, its dominant phylloplane fungal mat and culture filtrates of Alternaria alternata, Colletotrichum gloeosporioides, Paecilomyces lilacinus and Phoma chrysanthemicolashowed 44, 43 and 29 bioactive compounds, respectively. The major compounds identified were alcohols, alkaloids, carboxylic acids, chlorine containing, esters, ethers, hydrocarbons, iodine containing, oxalic acid, phenols & terpenoids. In a mass spectrum, each compounds were identified based on their retention time and peak area. The results of this study would offer a platform of using extracts of phylloplane fungiof Markhamia lutea as herbal alternative for some diseases.