Genetic diversity in the natural population of Ziziphus jujuba Miil
Nisar Uddin, Noor Muhammad, Mohammad Nisar, Jebran Muhammad, Niaz Ali
Genetic diversity is the material basis for the improvement of crops best of the current research work was done for the first time on natural genetic diversity based in total seed protein and phenotypic characteristic in 120 genotypes of Ziziphus jujube Mill. The genotypes was presented the utmost allelic variation for vigorous tree; 60 trees were less vigorous whereas 20were high 20 were vigorous and 20 is moderate types. Leaf type; 100% were with alternate, Leaf shape was totally ovate. All were green leaf color. Tomentose; 60 genotypes were dense, 40 were rare, 20 stems were red brown 20 light black, 50 brown, 10 were with purple and 10 were with grey stem with spines. Fruit color; 25,25 genotypes were with yellow red and red brown respectively, 25 were with brown, 25 were with red and 20 were with yelllow. Leaf margin; 80 entire leaf margin and 40 genotypes were with serrate margins. Fruit shape; 80 genotypes were round shaped fruit and 40 genotypes were with oval shaped fruits. Result: We have first time reported on protein profiling seed of Z. jujuba was carried out on 12% slab gel electrophoresis. 12 loci with molecular weight ranges from 10KDa to 180KDa were detected in Z. jujuba. The locus contribution toward genetic disagreement (LCTGD) genetic dissimilarity of Z. jujuba was 75.00%, L-6, 9 and L-11, were monomorphic in Z. jujuba and was treated as specie specific. L1-2, L-3, L-4, L-5, L-7, L-8, L-10 and L-12 were polymorphic. These alleles showed 33.333%, 50%, 70, 82.50%, 79.17%, 33.333, 70%, 79.17% and 29.166%% respectively. The intra-specific variation was limited and it was observed that SDS-PAGE alone did not exhibit high level of intra-specific variation; therefore, diverse germplasm based on SDS-PAGE is suggested to be acquired from various sources, preferably from center of diversity to build a broad based gene pool with maximum variability.