Effects of various plant growth regulators and ampicillin activity on in vitro propagation of Plumbago zeylanica: An important medicinal plant of south India-Kolli hills
Dr. PF Steffi, PF Mishel, Dr. B Thamilmaraiselvi, Dr. E Priya
Plumbago zeylanica belongs to the family Plumbaginaceae grows widely in India and has many medicinal values. The aim of this study is to produce one of the valuable medicinal plants (Plumbago zeylanica) in large quantities through plant tissue culture technique. In-vitro propagation was attempted through micro propagation and direct organogenesis. In Micro propagation, Nodal explants were used and micro propagation was achieved using BAP and KIN with various concentrations. Nodal explants were produced at a concentration of 3 mg/l BAP with 10 % of Coconut Water and 5 mg/l of Ampicillin. With reference to direct organogenesis, 4 mg/l BAP produced maximum results from Internodal explants. The final survival rate of hardening percentage was ranged from 85 - 90 obtained from various explants. The highest percentage of the survival was 90% were observed in nodal explants after four weeks.