Chenopodium murale Linn. is an evergreen annuals herb, and can be found throughout the year. Chenopodium murale are thought to possess therapeutic effects on many illnesses in worldwide. Present study was aimed to investigate an antioxidant, cytotoxic activities and phytochemical analysis of Chenopodium murale ethanol extract. The antioxidant activities of the extracts of Chenopodium murale were determined with methods such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity, hydrogen peroxide (H₂O₂) and superoxide (NBT) radical scavenging activity, determination of total phenolic content and total flavonoid contents. The binding action of the DPPH, ABTS, H₂O₂ and NBT radicals scavenging activity increases with concentration of the extracts. Methanol extract exhibited higher cytotoxic activity against brine shrimp. The total phenolic and flavonoid content estimated was 500 mg/g Gallic acid equivalent and 19160 mg/g rutin equivalent respectively. Results of the present study suggest that Chenopodium murale extract can be an alternative to synthetic antioxidant, anticancer and antibiotic agents.