Efficiency of Phosphate solubilizing bacteria isolated from different regions in dissolving of the insoluble phosphate and the activity of phosphatase enzyme
Mohamed Saeed Haran, AbdalZahra Taha Thaher
Ten isolates of Phosphate solubilizing bacteria were isolated from the soil of the rhizosphere for the plants cultivated in different locations of southern Iraq, the study of the culturing, microscopic and biochemical traits of these isolates, as well as testing the efficiency of these isolates in dissolving the phosphates in the liquid culture media, the activity of phosphatase enzyme, solubility index, their production of total acidity and reduce the reaction degree of the liquid media. The results showed that the isolates belonged to the Bacillus genus. B6 isolates were the most efficient isolates in dissolving the phosphate amounted to (36.6 mg P. L-1) and solubility index amounted to (3.428), as well as their superiority in reducing the pH for the growth media from 7 to 5.6, their production to total acidity of (1.24 Meq.L-1). The efficacy of the acid and alkaline phosphatase enzyme was (2.48 and 1.13 IU.L-1), respectively.