Kinetic and inhibitory effect of Silene arenosa extracts against pancreatic α amylase
Adil Shah, Mushtaq Ahmed, Nadia Mushtaq, Nila sher, Rahmat Ali Khan, Mehtab Alam
Background: This study examines the inhibition of alpha amylase (α-amylase) via using Silene arenosa water extract. To the best of our knowledge, there are no previous studies on the α-amylase inhibition activities of S. arenosa extracts. For this aim, S. arenosa extracted with water by maceration method and their α-amylase enzyme inhibition activity was performed. Methodology/Principal Findings: A maximum inhibition 74 and 88% byS. arenosa and acarbose of α-amylase activity was observed at fixed substrate concentration (20 mg). Kinetic studies indicate that the inhibition caused by S. arenosa was mixed type, i.e. Km increased from 18.7 to 22.5 mg (108.4-150.8%) while Vmax decreased from 0.022 to 0.004 mg (55.1-91.8%) in a concentration dependent manner. In α-amylase Kiapp was found to increase from 5.4 to 5.05 mg (14.2–19.8%) while Vmaxiappincrease from 0.01 to 0.016 µg (25–100%) with increase of substrate from (10–30 mg). Ki was estimated to be 5.4 mg while the Km(dissociation constant of α-amylase-potato starch-S. arenosa complex α-amylase-S. arenosa complex and potato starch) was 12.5mg. Thecalculated IC50 for S. arenosa and standard drug acarbose against α-amylase were 55 and 44 mg, respectively at constant substrate concentration (20 mg). Conclusions/Significance: The present results suggest that S. arenosa inhibit α-amylase enzyme activity and can be used as therapeutic agent for the treatment of diabetes-II.