International Journal of Botany Studies

International Journal of Botany Studies


International Journal of Botany Studies
International Journal of Botany Studies
Vol. 4, Issue 4 (2019)

Effect of solvent, time and temperature on the some chemical properties of Salep tuber (Anacamptis Collina)


Saadatian M, Asiaban K

To investigate the effects of solvent type (ethanol, methanol, acetone and water), methanol concentration (20-95% v/v) time (2-5 hours) and temperature (37-62 °C) on the extraction of total phenolic compounds (TPC), total flavonoid compounds (TFC), antioxidant capacity and carbohydrate solutes of Salep tuber (Anacamptis Collina) using a single factor experiments. all the studied extracting condition showed significant effect (p<0.05) on TPC, TFC, antioxidant capacity and carbohydrate solutes. On the basis of the parameters, the best extraction conditions were 95% methanol for 5 hours extraction at 50 °C. According to these optimized condition, high content of TPC, TFC, antioxidant capacity and carbohydrates of Salep tuber extracts were obtained with value of 73.15 GAE/100g, 178 QE/ 100g, 93% and 31.18 mg/g DW respectively. To obtain more data and accuracy of optimization should be investigating another extraction methods and condition for future.
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How to cite this article:
Saadatian M, Asiaban K. Effect of solvent, time and temperature on the some chemical properties of Salep tuber (Anacamptis Collina). International Journal of Botany Studies, Volume 4, Issue 4, 2019, Pages 71-77
International Journal of Botany Studies