Phytochemical analysis of selected Nepalese medicinal plants, Screening for Antioxidant activity and Determination of Total Phenol and Total Flavonoid Content
Mamita Ghimire, Sumitra Shrestha, Dr. Sushil Panta, Rashmi Thapa
Background: Oxidative stress has been demonstrated to participate in the progression of diabetes including impairment of insulin action and elevation of the complication incidences. Antioxidants have already shown to be prospective in the treatment of diabetes both type I and type II diabetes. Methods: The study was conducted to determine the total phenolic and flavonoid content of the ethanolic extracts of four Nepalese medicinal plants by the Folin Ciocalteu reagent and Aluminium chloride colorimetric method respectively. The antioxidant activity of the ethanolic extract was determined by the DPPH (2, 2-diphenyl-1-picryl-hydrazyl) radical scavenging method using ascorbic acid as the positive control was measured at different concentration (1, 10, 100 µg/mL). Results: The phytochemical analysis revealed presence of phenols and flavonoids in Magnolia grandiflora and Mangifera indica. The total phenolic content and flavonoid content were 353.81± 0.84 mgGAE/g and 310.54±0. 0 mgQE/g dry extract weight for unripe Mangifera indica respectively. The extracts of unripe Mangifera indica showedantioxidant activity with the IC50 values of 3.87 μg/mL compared with the standard ascorbic acid IC50 value of 4.22 μg/mL. Conclusion: In this study showed potential antioxidant, with present of Flavonoids and Phenols. These data are important because they contribute to the recording of traditional knowledge used for the antioxidant activities.