Murungai poo, the flowers of Moringa oleifera Lam. are highly used in various traditional medical systems. The flowers contain bioactive phytochemicals and possesses promising antibacterial, antifungal, anti-larval, antioxidant, anti-inflammatory and anticancer properties. The authenticated flowers of M. oleifera were subjected to detailed macro microscopic and quantitative profiling, physico-chemical analysis, phytochemical screening and HPTLC. The flowers are zygomorphic. TS through pedicel was circular in outline, shows outer epidermis, middle cortex having abundant starch grains and inner pith region; TS through calyx and corolla showed numerous trichomes and mesophyll tissues but rosette crystals were present only in calyx. The powdered drug contained thick walled trichomes, parenchyma cells, vessels, pollen grains, rosette crystals and starch grains. The phytochemical screening gave substantial values of different parameters of the floral drug. The plants parts were subjected to TLC and HPTLC studies with CAMAG TLC and diagonostic peaks were recorded under UV 254 nm, 366 nm and 575 nm. This detailed pharmacognostic characterization will prove to be an exhaustive record for future reference and research on this herbal drug.